Lambda Location #1Chapter 2: "A Red Letter Day". Just inside the entrance Alyx opens for you, slightly to the right; you'll likely pick it up automatically as you walk in.
Lambda Location #2Chapter 3: Route Kanal. Just after that room, in the first radioactive water area, cross the pipes to the right side to reach it.
Lambda Location #2Chapter 3: Route Kanal. After jumping across to the building where you wait for a train, it's to your left as you enter.
Lambda Location #2Chapter 3: Route Kanal. In the air vent you have to crawl through almost immediately after; there's no way to miss it.
Lambda Location #2Chapter 3: Route Kanal. Hop on top of the red van to the left, just before your second barnacle encounter, and duck into the rubble.
Lambda Location #2Chapter 3: Route Kanal. Through the grate below the room you enter after being shot at by a helicopter for the first time; take the ladder to your right down to it.
Lambda Location #2Chapter 3: Route Kanal. Continue underneath the big orange pipe instead of dropping into the dark hole to the right at the end of it.
Lambda Location #2Chapter 3: Route Kanal. Stay on the upper level of the curved room you climb a ladder into, all the way to the end.
Lambda Location #9Chapter 4: Water Hazard. Head up the left side of the V-shaped canal with the reddish-orange buildings, towards the far end.
Lambda Location #9Chapter 4: Water Hazard. Just past the barn, where a dropship drops Combine in your path, the leftmost of two pipe entrances on the right is open; use a barrel to climb up and inside.
Lambda Location #9Chapter 4: Water Hazard. Just before the big red barn, once the water clears up again, stop the boat and look right for a ladder leading up to it.
Lambda Location #9Chapter 4: Water Hazard. In an easy-to-miss square room with scaffolding on the left, just before the stretch where you cross the top of some pipes.
Lambda Location #9Chapter 4: Water Hazard. There are two chances at this one: in front of some stairs you climb to jump across containers, or (if you miss that) underneath a bridge with a parked train, just past the point where the helicopter returns fully repaired.
Lambda Location #9Chapter 4: Water Hazard. Just after entering an area filled with rubble, solve the small counter-weight basket puzzle before bashing through the wood to continue.
Lambda Location #9Chapter 4: Water Hazard. There are two chances at this one: a hard-to-spot ladder up to a corner platform between two turbines just before the gate opens, or (if you miss that) a small grate below the second rocket-launching car later on.
Lambda Location #9Chapter 4: Water Hazard. At the very top of the big red barn, in a mechanism jammed with a barrel that only the crowbar can free; the items that drop outside are the cache.
Lambda Location #9Chapter 4: Water Hazard. In a dead-end area with a windmill, off to the right at a fork in the canal.
Lambda Location #9Chapter 4: Water Hazard. Just after that, make an almost U-turn to the left into a dark area, avoiding the boards marked with red X's.
Lambda Location #19Chapter 5: Black Mesa East. At the ladder you use to get out of the boat for the last time; you likely picked it up automatically just by passing near it.
Lambda Location #20Chapter 6: "We Don't Go to Ravenholm...". In the area with the car traps, ride a car up and jump over the fence marked with the symbol.
Lambda Location #20Chapter 6: "We Don't Go to Ravenholm...". Take a left turn instead of a right after dropping down to the road and climbing the ladder at the far end.
Lambda Location #20Chapter 6: "We Don't Go to Ravenholm...". Above the exit as you step out into daylight at the very end of the chapter.
Lambda Location #20Chapter 6: "We Don't Go to Ravenholm...". Hop on top of one of the green generators, just after first meeting Father Grigori, and go through the vent.
Lambda Location #24Chapter 7: Highway 17. Inside the first building in this map segment, next to the first thumper.
Lambda Location #24Chapter 7: Highway 17. Behind a fenced fuel tank across the road from the billboard used for Targetted Advertising; shooting below the tank to break the crate open works without going inside.
Lambda Location #24Chapter 7: Highway 17. Stick to the left wall entering this segment; it's in the rocky area with some headcrabs, just before the hill leading up to the famous bridge.
Lambda Location #24Chapter 7: Highway 17. In the red building on the left, just after your first jump and before your first tunnel.
Lambda Location #24Chapter 7: Highway 17. In a blue van on the left, just past the pile of wrecked cars where you fight your second gunship.
Lambda Location #29Chapter 8: Sandtraps. Partway across the antlion beach, toss a grenade or rocket up onto the white building above the second red building to knock it loose.
Lambda Location #29Chapter 8: Sandtraps. In the house with the car batteries, look up into the small gap above the bathroom ceiling and pull it down with the Gravity Gun or a thrown object.
Lambda Location #31Chapter 9: Nova Prospekt. After unplugging the machine the Vortigaunt is strapped to and opening the gate, continue straight ahead through the door instead of turning; the symbol is directly across the room.
Lambda Location #31Chapter 9: Nova Prospekt. In a room behind locked doors just before the next map segment, in the A5 sector; smash the boards blocking the window to get in.
Lambda Location #31Chapter 9: Nova Prospekt. Jump the railing to the right in the laundry room.
Lambda Location #34Chapter 9a: Entanglement. On top of the Dr. Breen's Private Reserve vending machine on the left, while waiting for Alyx to open the first gate.
Lambda Location #35Chapter 10: Anticitizen One. Tucked in a corner as you make a U-turn back onto the road, after passing through a sidewalk with windows on your right.
Lambda Location #35Chapter 10: Anticitizen One. Behind a gate reached by going up the stairs instead of down the hall towards the next map segment, past the turret-guarded hole in the wall.
Lambda Location #35Chapter 10: Anticitizen One. Tucked behind a large reddish building slightly above the exit, at the top of a slow-moving lift you ride and climb.
Lambda Location #35Chapter 10: Anticitizen One. In a small red shack on the left in the alleyway where Combine start appearing in windows; remove the metal prop blocking it.
Lambda Location #35Chapter 10: Anticitizen One. In a tiny hole on your right, past some zombies fighting Combine at the bottom of a sewer-like area; suck the supplies out with the Gravity Gun.
Lambda Location #35Chapter 10: Anticitizen One. Immediately outside, pile up some objects to get behind the fence behind the blue dumpster on the right.
Lambda Location #35Chapter 10: Anticitizen One. Step outside through a wood-rimmed doorway (instead of continuing left up the stairs) after a large combine-vs-zombie battle, then look in the dark area on your left.
Lambda Location #35Chapter 10: Anticitizen One. In the room just before the first bridge you lower yourself, on the left; it's hard to miss.
Lambda Location #35Chapter 10: Anticitizen One. Just past the previous cache, continue past three metal pylons to the end instead of climbing the nearby ladder.
Lambda Location #35Chapter 10: Anticitizen One. At the bottom of a stairwell full of catwalks, below the metal door you remove a bar from to continue.
Lambda Location #45Chapter 11: "Follow Freeman!". In a hallway almost directly in your path, just before the final map segment where a strider chases you down a tunnel.